Targeted sequencing enables efficient analysis of customer-selected genes through high-throughput sequencing technology. For several or hundreds of genes, targeted sequencing exhibit higher sensitivity and specificity, as well as higher coverage, and yield high quality data.Targeted sequencing has been widely used in agrigenome research, providing new ideas and help for molecular breeding. It also has important applications for food safety, animal health, forensics, and environmental studies.

CD Genomics provides high quality targeted sequencing services that use probe hybridisation to enrich DNA sequences in exonic regions, based on high throughput sequencing technology to drill down to specific genes, coding regions, or chromosomal segments to rapidly and accurately study the genome and discover mutations associated with protein functional variation. Our targeted sequencing services help researchers to identify point mutations, insertions or deletions (INDELS), copy number variations (CNVs) and gene rearrangements. Our targeted sequencing is primarily used for the study of large samples and small region variants, and is widely used in the detection of low frequency mutations in plant and animal genomes.

Features of targeted sequencing technology

• Targeted gene sequencing produces smaller, easier-to-manage data sets that are easier to analyze.
• Technology is more targeted, with deeper coverage, higher data accuracy, simpler, more economical and more efficient.
• Highly flexible, with custom primers to detect any region of interest in the genome.
• Cost-effective and suitable for analysis of large sample sizes.

Advantages of targeted sequencing

• Deep sequencing of key genes or regions of interest, used to identify rare variants.
• Provides accurate, easily interpretable results that identify variants at low allele frequencies (down to 5%).
• Efficiently identify novel or genetic mutations in species.

Our targeted sequencing can be used in the following studies

• Discovery of genetic biomarkers associated with disease states in plants and animals.
• Characterization of genetic correlation to a specific phenotype.
• Genotyping and classification of eukaryotic or prokaryotic cell lines.
• Designing genetic assays.

Instructions for providing samples

• Accepted sample types
• Purified genomic DNA
• Frozen cell clumps (mammalian cells or bacterial cells)
• PCR amplicons
• Bacterial communities
• Tissue samples
• Animal blood

Targeted sequencing workflow

Fig 2. Targeted sequencing workflow

Our advantages and features

• We provide a full range of services from DNA extraction to comprehensive data analysis.
• High turnaround rate, our advanced sequencing platform and experienced technical team will provide you with the best service and improve your research efficiency.
• Rich experience in project synthesis design to meet multi-species and multi-omics sequencing research.
• Cost-effective, with the ability to mix and match a large number of samples at the same time.
• We provide you with technical support services to discuss the progress of your project and solve technical problems.

CD Genomics has advanced sequencing platforms and self-developed synthesis platforms, equipped with strict standardized service procedures to ensure complete quality control management of each experimental batch to ensure that we provide you with good and accurate data results. If you are interested in us, please feel free to contact us.